This study investigated the use of vegetable oil to cleanse nitrate fr
om groundwater: The hypothesis on which the study was based was that o
il trapped in the sail matrix would form an immobile organic zone thro
ugh which water flows; nitrate in the water would be removed as microb
ial denitrifiers utilized the oil as a carbon source. Laboratory studi
es show that the procedure has merit. Both corn and soybean oil rapidl
y stimulated native bacteria to remove nitrate from water in static an
aerobic bioreactors. Soybean oil/water mixtures ranging from 0.8 to 12
.5% oil in water were tested and all were effective at supporting deni
trification. Also, denitrification occurred rapidly at nitrate concent
rations up to 2000 ppm NO3-N. In addition to studies with static biore
actors, soil column studies were conducted. Soybean oil injected into
2.5 x 34 cm columns containing aquifer matrix or sand removed nitrate
from flowing water Flow rates of Icp to 600 mL/day were successful. In
nocuous vegetable oil may provide the basis for a simple and inexpensi
ve method of nitrate removal from contaminated groundwater.