HISTOENZYMIC AND HISTOCHEMICAL ALTERATIONS IN PHYSIOPATHOLOGY OF CNS IN RABIES

Histoenzymic and histochemical study was carried out in cryostat secti ons of cerebellum, hippocampus, cerebrum, medulla oblongata, pens and spinal cord of buffalo calves experimentally infected with street rabi es virus wherein histoenzymic activity of nonspecific esterases, succi nic dehydrogenases, acetyl cholinesterase, mono amino oxidase, adenosi ne triphosphatase, acid phosphatase, alkaline phosphatase and lactic d ehydrogenase were studied in the cryostat sections of central nervous system. Significant loss of histoenzymic activity of esterases indicat ed loss of lipid metabolism which was supported by increased histochem ical detection of lipid droplets. Decreased activity of dehydrogenases and oxidases indicated neuronal degeneration and decreased protein sy nthesis respectively. Decrease in the cholinergic function was indicat ed due to progressive decrease in, the acetylcholinesterase level in n eurons of central nervous system. The histoenzymic and histochemical a lterations depicted definite neurological damage in the present experi mental rabies produced by street rabies virus.