ELECTROPHYSIOLOGICAL EFFECTS OF BACILLUS-SPHAERICUS BINARY TOXIN ON CULTURED MOSQUITO CELLS

Authors
COKMUS C DAVIDSON EW COOPER K
Citation
C. Cokmus et al., ELECTROPHYSIOLOGICAL EFFECTS OF BACILLUS-SPHAERICUS BINARY TOXIN ON CULTURED MOSQUITO CELLS, Journal of invertebrate pathology, 69(3), 1997, pp. 197-204
Citations number
31
Categorie Soggetti
Zoology
Journal title
Journal of invertebrate pathology → ACNP
ISSN journal
00222011
Volume
69
Issue
3
Year of publication
1997
Pages
197 - 204
Database
ISI
SICI code
0022-2011(1997)69:3<197:EEOBBT>2.0.ZU;2-9
Abstract
The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins of Bacillus sphaericus were invest igated on cultured Culex quinquefasciatus cells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were c orrelated with increasing native toxin concentration. The 42- or 51-kD a cloned toxin alone at 50 IJ;mu g/ml reduced the resistance. Electrop hysiological effects occurred before any changes were visible by phase -contrast microscopy. (C) 1997 Academie Press.