IMMUNOELECTROCHEMICAL ASSAY IN COMBINATION WITH HOMOGENEOUS ENZYME-LABELED ANTIBODY CONJUGATION FOR RAPID DETECTION OF SALMONELLA

An immunoelectrochemical assay in combination with homogeneous enzyme- labeled antibody conjugation was developed for rapid detection of Salm onella. The assay was performed by mixing alkaline phosphatase linked anti-Salmonella (APLAS) with Salmonella in a solution. The Salmonella- APLAS conjugate separated by polycarbonate membrane filtration was inc ubated with a phenyl phosphate substrate to produce phenol. The concen tration of Salmonella cells was determined by measuring phenol oxidati on peak current using differential pulse voltammetry at a renewable ca rbon paste electrode. This assay could be completed within two hours w ith a detection limit of 5 x 10(3) cells/mL. A linear response was fou nd for Salmonella between 5 x 10(3) and 1 x 10(6) cells/mL.