COMPETITIVE-INHIBITION OF LENS-CULINARIS L. COPPER AMINE OXIDASE BY AMILORIDE, P-AMINOBENZAMIDINE, CLONIDINE, 4',6-DIAMIDINO-2-PHENYLINDOLEAND GABEXATE MESYLATE - A COMPARATIVE-STUDY
L. Ercolini et al., COMPETITIVE-INHIBITION OF LENS-CULINARIS L. COPPER AMINE OXIDASE BY AMILORIDE, P-AMINOBENZAMIDINE, CLONIDINE, 4',6-DIAMIDINO-2-PHENYLINDOLEAND GABEXATE MESYLATE - A COMPARATIVE-STUDY, Journal of enzyme inhibition (Print), 13(6), 1998, pp. 465-471
The competitive inhibition of Lens culinaris,is L. copper amine oxidas
e by amiloride (K-i = 4.1 x 10(-4) M), p-aminobenzamidine (K-i = 6.0 x
10(-4) M), clonidine (K-i = 5.0 x 10(-4) M), 4',6-diamidino-2-phenyli
ndole (DAPI; K-i = 1.9 x 10(-5) M) and gabexate mesylate (K-i = 2.5 x
10(-4) M) has been investigated, at pH 7.0 and 25 degrees C. The affin
ity of p-aminobenzamidine, clonidine and DAPI for plant and mammalian
copper amine oxidase is closely similar. However, values of K-i for am
iloride and gebexate mesylate binding to swine kidney copper amine oxi
dase are lower than those observed for inhibitor binding to Lens culin
aris L. copper amine oxidase. Thus, amiloride and gabexate mesylate ma
y represent useful model compounds for the development of selective in
hibitors of mammalian copper amine oxidase, which may be important in
view of the potential use of plant copper amine oxidase as drugs.