DISCRETE DISTRIBUTION OF THE NEUROPEPTIDE-Y Y-5 RECEPTOR GENE IN THE HUMAN BRAIN - AN IN-SITU HYBRIDIZATION STUDY

The present study investigated the regional distribution of putative ' food-intake'-related neuropeptide Y Y-5 receptor gene using cRNA in si tu hybridization in various regions of the normal control post-mortem human brain. Interestingly, significant levels of Y-5 receptor express ion were detected in the hypothalamus: the arcuate nucleus being parti cularly enriched compared to other hypothalamic nuclei. Surprisingly, strong hybridization signals were also noted in the stratum granulosum of the dentate gyrus contrasting with lower levels of Y-5 receptor tr anscripts in other regions of the hippocampal formation. The cerebral cortex, basal ganglia and thalamus were not enriched with Y-5 receptor mRNA. It thus appears that the expression of the Y-5 receptor gene in the human brain is rather restricted with enrichment in areas consist ent with the involvement of this receptor type in the modulation of ap petite and seizures. (C) 1998 Elsevier Science B.V. All rights reserve d.