KINETICS OF INHIBITION OF PENAEUS-PENICILLATUS ACID-PHOSPHATASE BY BROMOACETIC ACID

The kinetics of inhibition of penaeus penicillatus acid phosphatase by bromoacetic acid has been studied. The results show that inhibition o f the enzyme by bromoacetic acid is a slow, reversible reaction. The m icroscopic rate constants for the reaction of inhibitor with the enzym e were determined. The presence of the substrate offers marked protect ion of this enzyme against inhibition by bromoacetic acid The above re sults suggest that the histidine residue is essential for activity and are situated at the active site of the enzyme.