DIAGNOSIS OF THE ANTIPHOSPHOLIPID SYNDROME - A PROPOSAL FOR USE OF LABORATORY TESTS

The presence of antiphospholipid (aPL) antibodies has been associated with thrombosis, pregnancy loss and thrombocytopenia in the antiphosph olipid syndrome (APS). The anticardiolipin and the lupus anticoagulant tests are frequently used to detect aPL antibodies. The anticardiolip in ELISA utilizes cardiolipin coated on polystyrene plates as antigen and is a very sensitive test but lacks specificity? since it can be po sitive in a number of infectious (such as syphilis, HIV) and autoimmun e diseases other than APS. In an effort to improve specificity, new EL ISA techniques that employ alternative antigens (such as beta(2)-glyco protein 1, particularly when coated onto oxidized microtiter plates or mixture of phospholipids) have been developed. Several investigators have reported that these new assays enable more specific determination of aPL antibodies and thus can be used more reliably for the diagnosi s and confirmation of APS. This article examines the results of those studies, including data that shows correlations of these assays with c linical manifestations of APS, and proposes a new protocol for the use of laboratory tests in the diagnosis of APS.