Ml. Herlocher et al., IMMUNOLOGICAL PROPERTIES OF PLAQUE PURIFIED STRAINS OF LIVE ATTENUATED RESPIRATORY SYNCYTIAL VIRUS (RSV) FOR HUMAN VACCINE, Vaccine, 17(2), 1999, pp. 172-181
Citations number
45
Categorie Soggetti
Veterinary Sciences",Immunology,"Medicine, Research & Experimental
Respiratory syncytial virus (RSV) causes severe lower respiratory trac
t disease in infants, young children, and the elderly. Efforts to deve
lop satisfactory live or inactivated vaccines have not yet been proven
successful. Our research focuses on the development of four purified
live attenuated RSV sub-type A human vaccine clones. Temperature sensi
tive (ts) and attenuated purified clones of either cold-adapted (ca) R
SV or high-passage (hp) RSV were administered intra-nasally (i.n.) to
BALB/c mice and tested for immunogenicity. All four clones produced si
gnificant anti-RSV F IgG(2a) and IgG(1) titres in the sera of mice, RS
V-specific neutralizing titres higher than those produced by their wil
d-type progenitor viruses, cytotoxic T-lymphocyte (CTL) activity, and
total protection against wild-type (wt) viral challenge. These purifie
d vaccine candidates await testing in humans to determine which contai
n the required balance between immunogenicity and attenuation. (C) 199
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