ISOLATION AND SEPARATION OF HMG PROTEINS AND HISTONES H1 AND H5 AND CORE HISTONES BY COLUMN CHROMATOGRAPHY ON PHOSPHOCELLULOSE

Authors
GARG LC REECK GR
Citation
Lc. Garg et Gr. Reeck, ISOLATION AND SEPARATION OF HMG PROTEINS AND HISTONES H1 AND H5 AND CORE HISTONES BY COLUMN CHROMATOGRAPHY ON PHOSPHOCELLULOSE, Protein expression and purification (Print), 14(2), 1998, pp. 155-159
Citations number
20
Categorie Soggetti
Biochemical Research Methods",Biology,"Biothechnology & Applied Migrobiology
Journal title
Protein expression and purification (Print) → ACNP
ISSN journal
10465928
Volume
14
Issue
2
Year of publication
1998
Pages
155 - 159
Database
ISI
SICI code
1046-5928(1998)14:2<155:IASOHP>2.0.ZU;2-6
Abstract
A single-step method to dissociate histones as well as nonhistone chro mosomal proteins from chicken erythrocyte chromatin and their separati on into histones H1, H5, core histones, and high mobility group protei ns by column chromatography on phosphocellulose is presented. NaCl at 2.0 RI is effective in dissociating both histones and nonhistone prote ins. The core histones elute as a complex. The pH is a critical factor in separating H5 from the core histones. (C) 1998 Academic Press.