P. Olson et al., HIGH-LEVEL EXPRESSION OF EUKARYOTIC POLYPEPTIDES FROM BACTERIAL CHROMOSOMES, Protein expression and purification (Print), 14(2), 1998, pp. 160-166
Citations number
18
Categorie Soggetti
Biochemical Research Methods",Biology,"Biothechnology & Applied Migrobiology
Attractive economics and short development timelines have often been c
ited as reasons for using bacteria to express eukaryotic proteins on a
commercial scale. Nevertheless, routine techniques for bacterial expr
ession of heterologous proteins are beset by a variety of technical an
d legal difficulties. In particular, the use of plasmids to express fo
reign proteins, popular promoter systems, protein fusion partners, and
histidine tags and the recovery of proteins from inclusion bodies are
affected by a host of issued patents. Chromosomally encoded leaderles
s fusions (CELF) offer a variety of technical and legal advantages ove
r existing bacterial expression systems. In this study, we show that C
ELF can be used to produce a wide assortment of eukaryotic proteins at
10-liter fermentation scale. (C) 1998 Academic Press.