ANTIFUNGAL METABOLITES (MONORDEN, MONOCILLIN-IV, AND CEREBROSIDES) FROM HUMICOLA-FUSCOATRA TRAAEN NRRL-22980, A MYCOPARASITE OF ASPERGILLUS-FLAVUS SCLEROTIA

The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a scl erotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the a ntifungal metabolites monorden, monocillin IV, and a new monorden anal og. Each metabolite produced a clear zone of inhibition surrounding pa per assay disks on agar plates seeded with conidia of A. flavus. Monor den was twice as inhibitory to A. flavus mycelium extension (MIC > 28 mu g/ml) as monocillin IV (MIC > 56 mu g/ml). Cerebrosides C and D, me tabolites known to potentiate the activity of cell wall-active antibio tics, were separated from the ethyl acetate extract but were not inhib itory to A. flavus when tested as pure compounds. This is the first re port of natural products from H. fuscoatra.