EVIDENCE FOR THE INTERPLAY BETWEEN TREHALOSE METABOLISM AND HSP104 INYEAST

Disruption of the HSP104 gene in a mutant which cannot accumulate treh alose during heat shock treatment caused trehalose accumulation (H. Iw ahashi, K. Obuchi, S. Fujii, and Y. Komatsu, Lett. Appl. Microbiol 25: 43-47, 1997). This implies that Hsp104 affects trehalose metabolism. Thus, me measured the activities of enzymes involved in trehalose meta bolism The activities of trehalose-synthesizing and -hydrolyzing enzym es are low in the HSP104 disruption mutant during heat shock, This dat a is correlated with intracellular trehalose and glucose levels observ ed in the HSP104 disruption mutant. These results suggest that during heat shock, Hsp104 contributes to the simultaneous increase in both ac cumulation and degradation of trehalose.