EXPEDITIOUS SYNTHESIS OF A NEW HEXASACCHARIDE USING TRANSGLYCOSYLATION REACTION CATALYZED BY BACILLUS (1-]3),(1-]4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE

Authors
VILADOT JL STONE B DRIGUEZ H PLANAS A
Citation
Jl. Viladot et al., EXPEDITIOUS SYNTHESIS OF A NEW HEXASACCHARIDE USING TRANSGLYCOSYLATION REACTION CATALYZED BY BACILLUS (1-]3),(1-]4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE, Carbohydrate research, 311(1-2), 1998, pp. 95-99
Citations number
12
Categorie Soggetti
Chemistry Applied","Chemistry Inorganic & Nuclear",Biology
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
311
Issue
1-2
Year of publication
1998
Pages
95 - 99
Database
ISI
SICI code
0008-6215(1998)311:1-2<95:ESOANH>2.0.ZU;2-2
Abstract
Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a r ecombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with thi s substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3G lc beta 4Glc beta 4Glc beta 3Glc in 20% yield. (C) 1998 Elsevier Scien ce Ltd. All rights reserved.