AMPEROMETRIC GLUCOSE ENZYME ELECTRODE BY IMMOBILIZING GLUCOSE-OXIDASEIN MULTILAYERS ON SELF-ASSEMBLED MONOLAYERS SURFACE

A novel and robust amperometric enzyme electrode for the determination of glucose was constructed by immobilizing glucose oxidase (GOD) and Os(bpy)(2)Cl-poly(4-vinyl)pyridine (Os-PVP) complex multilayers on thi ol self-assembled monolayers surface. The apparent Michaelis-Menton co nstant K-m' increased with increasing the number of Os-PVP/GOD multila yers. The concentration range of linear response and detection limit w ere 0.1-10 and 0.05 mM, the interference of ascorbic acid and uric aci d were eliminated by the presence of SAMs and the enzyme electrodes we re stable over 3 weeks. The preparation technique may be useful for co ntrolling the performance of multilayer enzyme electrodes by changing the enzyme content. (C) 1998 Elsevier Science B.V. All rights reserved .