REGIONAL LEVELS OF PHOSPHOLIPASE C-GAMMA AFTER FLUID PERCUSSION BRAININJURY IN THE RAT

Levels of PLC gamma, a phospholipase C (PLC) isozyme, were significant ly increased in the cytosol in the injured left cortex (LC) at 5, 30 a nd 120 min after brain injury. In the same site, although levels of me mbrane PLC gamma did not alter at 5 and 30 min, they were found to be decreased at 2 h after brain injury. Ln general, the levels of both cy tosolic and membrane PLC gamma were unaltered in the contralateral rig ht cortex (RC), ipsilateral left hippocampus (LH) and contralateral ri ght hippocampus (RH) between 5 and 120 min after brain injury. These r esults suggest that, in addition to well-proposed excitatory neurotran smitter-receptor systems, increased levels of PLC gamma may also contr ibute to alterations in PIP2 signal transduction pathway, particularly in the greatest injury site (LC) after lateral FP brain injury. (C) 1 998 Published by Elsevier Science B.V. All rights reserved.