SHORT TANDEM REPEAT (STR) LOCUS HUMD8S306 IN A LARGE POPULATION-SAMPLE FROM GERMANY

Applied DNA typing in medico-legal investigations, in criminalistic pr actice, and in paternity cases often relies on high inclusion and excl usion probabilities. For that reason, the short autosomal tandem repea t locus D8D306 was validated for forensic use and incorporated into a nonoverlapping multiplex reaction with HUMDHFRP2 and HUMCD4. The allel e frequencies of D8S306 in four different regions of Germany (n = 1220 alleles) were determined for use in a population database; the allele distributions did not significantly deviate from each other. The here rozygosity of D8S306 is 83 %, expected exclusion chance in stain cases is 96% (paternity cases: 69%), the lowest amount of sucessfully ampli fied DNA was 30 pg. The alleles are in Hardy-Weinberg equilibrium (exa ct test).