G. Asmundsson et al., EFFECTS OF VENTROLATERAL MEDULLARY NMDA-RECEPTOR ANTAGONISM ON BIOGENIC-AMINES AND PRESSOR-RESPONSE TO MUSCLE-CONTRACTION, Neuroscience research, 32(1), 1998, pp. 47-56
Effects of D(-)2-amino-7-phosphonohepatanoic acid (AP-7), an N-methyl-
D-aspartic acid (NMDA) receptor antagonist, administered into rostral
ventrolateral medulla (RVLM) on changes in mean arterial pressure (MAP
), heart rate (HR), extracellular levels of serotonin (5-HT), dopamine
(DA), and norepinephrine (NE) during static muscle contraction were i
nvestigated in anesthetized rats. Tibial nerve stimulation-evoked musc
le contraction increased MAP and HR by 25 +/- 3 mmHg and 29 +/- 4 bpm,
respectively. Microdialysis of AP-7 (1 mu M) into the RVLM for 30 min
attenuated the contraction-evoked cardiovascular responses with simil
ar developed muscle tensions, without baseline HR or blood pressure ch
anges. Administration of AP-7 into the caudal ventrolateral medulla ha
d no effect on MAP or HR responses during contraction. Muscle contract
ion increased extracellular 5-HT in the RVLM by 144 +/- 35%, DA by 104
+/- 15% and NE by 62 +/- 12%. Perfusion of AP-7 for 30 min into the R
VLM attenuated contraction-evoked increases in monoamines, concomitant
to attenuating cardiovascular responses. Results demonstrate that NMD
A-receptor blockade within the RVLM, but not the CVLM, inhibits cardio
vascular responses during muscle contraction. Furthermore, NMDA recept
or antagonism within the RVLM results in a decrease of biogenic amine
release during muscle contraction, suggesting that extracellular bioge
nic amine concentrations are modulated by NMDA receptors. (C) 1998 Els
evier Science Ireland Ltd. All rights reserved.