ABERRATIONS OF CHROMOSOME-1 AND CHROMOSOME-17 IN 6 HUMAN OSTEOSARCOMACELL-LINES USING DOUBLE-TARGET FLUORESCENCE-IN-SITU-HYBRIDIZATION

Analysis of six human osteosarcoma cell lines was performed by using d ouble-target fluorescence in situ hybridization (FISH). FISH was appli ed to interphase nuclei, not to metaphase chromosomes. In this study, numerical aberrations of chromosomes 1 and 17 or structural chromosoma l aberrations of chromosome arm 1p or 17p, in which it has been sugges ted that there are one or more tumor suppressor genes in various malig nant tumors, were examined with this technique. All six of the human o steosarcoma cell lines studied had extra copies of chromosomes 1 and 1 7. A high frequency of deletions (>60%) in chromosome 1 was found in t wo cell lines and deletions of chromosome 17 were found in one cell li ne. (C) Elsevier Science Inc., 1998.