NUMERICAL ABNORMALITIES OF CHROMOSOME-1 AND CHROMOSOME-10 IN ENDOMETRIAL ADENOCARCINOMA - FLUORESCENCE-IN-SITU-HYBRIDIZATION ANALYSIS OF 23ARCHIVAL PARAFFIN-EMBEDDED SAMPLES

Retrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei is olated from formalin-fixed paraffin-embedded tissue. We examined 23 ar chival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosome s 1 and 10 were detected in 39% of the case analyzed, and the frequenc y of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involvi ng chromosomes 1 and 10 with endometrial carcinoma, as reported by oth er studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation. (C) Elsevier Scien ce Inc., 1998.