Sl. Abidi, SEPARATION PROCEDURES FOR PHOSPHATIDYLSERINES, Journal of chromatography B. Biomedical sciences and applications, 717(1-2), 1998, pp. 279-293
Citations number
69
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
This paper reviews working procedures for the separation of phosphatid
ylserines (PS) in complex sample matrices. It begins with an introduct
ory overview of important aspects of PS involvement in cellular lipid
biochemistry. The main body of the review describes various procedures
for the extraction, isolation, purification, and separation of the PS
class and its molecular species in tissue samples. Published high-per
formance liquid chromatographic methods are summarized to demonstrate
the variability and versatility of separation techniques. Factors infl
uencing normal-phase and reversed-phase separations are delineated. Th
e last section covers selected chemical derivatization procedures usef
ul for enhancing the separation efficiency and detection sensitivity a
nd specificity. (C) 1998 Elsevier Science B.V. All rights reserved.