SEPARATION PROCEDURES FOR PHOSPHATIDYLSERINES

This paper reviews working procedures for the separation of phosphatid ylserines (PS) in complex sample matrices. It begins with an introduct ory overview of important aspects of PS involvement in cellular lipid biochemistry. The main body of the review describes various procedures for the extraction, isolation, purification, and separation of the PS class and its molecular species in tissue samples. Published high-per formance liquid chromatographic methods are summarized to demonstrate the variability and versatility of separation techniques. Factors infl uencing normal-phase and reversed-phase separations are delineated. Th e last section covers selected chemical derivatization procedures usef ul for enhancing the separation efficiency and detection sensitivity a nd specificity. (C) 1998 Elsevier Science B.V. All rights reserved.