CHROMOSOMAL-ABERRATIONS IN COUPLES UNDERGOING INTRACYTOPLASMIC SPERM INJECTION - INFLUENCE ON IMPLANTATION AND ONGOING PREGNANCY RATES

Objective: To determine the incidence of chromosomal aberrations in co uples undergoing intracytoplasmic sperm injection (ICSI) and their inf luence on subsequent implantation and ongoing pregnancy rates. Design: Prospective study. Setting: Fertility center. Patient(s): Candidates for ICSI. Intervention(s): Chromosomes were trypsin-banded in 2,280 pa tients. In all cases, 10 metaphases were karyotyped. Sex chromosome an alysis was performed in 10 additional metaphases. When apparent chromo somal aberrations were detected, 100 metaphases were analyzed. Main Ou tcome Measure(s): Implantation and ongoing pregnancy rates in couples with a chromosomal disorder. Results: A chromosomal abnormality was de monstrated in 7.2% of all couples. Among the male partners, 4.48% had aberrations. Autosomal aberrations were present in 2.96%, and numerica l or structural sex chromosome abnormalities were found in 1.52%. Amon g the female partners, numerical or structural abnormalities were docu mented in 9.79%. Only 2.32% of the female partners had autosomal struc tural abnormalities. Numerical or structural anomalies involving sex c hromosomes were found in 7.47%. Implantation rates of 9.4% and 16.3% p er embryo were observed in female partners with sex chromosome mosaici sm and autosomal aberrations, respectively. In male partners, the resp ective rates were 3.8% and 23.1%. Conclusion(s): The incidence of chro mosomal disorders in couples seeking ICSI treatment is considerable, e specially minor mosaicism (<10%) of sex chromosomes in the female part ners. Preliminary data indicate a low implantation rate in couples wit h minor mosaicism of sex chromosomes. (Fertil Steril(R) 1998;70:933-7. (C) 1998 by American Society for Reproductive Medicine.).