VIABILITY OF CHLAMYDIA-TRACHOMATIS IN FALLOPIAN-TUBES OF PATIENTS WITH ECTOPIC PREGNANCY

Citation
Hc. Gerard et al., VIABILITY OF CHLAMYDIA-TRACHOMATIS IN FALLOPIAN-TUBES OF PATIENTS WITH ECTOPIC PREGNANCY, Fertility and sterility, 70(5), 1998, pp. 945-948
Citations number
4
Categorie Soggetti
Obsetric & Gynecology
Journal title
ISSN journal
00150282
Volume
70
Issue
5
Year of publication
1998
Pages
945 - 948
Database
ISI
SICI code
0015-0282(1998)70:5<945:VOCIFO>2.0.ZU;2-M
Abstract
Objective: To use standard molecular methods to define the prevalence and metabolic characteristics of Chlamydia trachomatis during infectio n of fallopian tubes in women with ectopic pregnancies. Design: Polyme rase chain reaction (PCR)- and reverse transcription-PCR (RT-PCR)-base d assessment of presence of chlamydial DNA and various RNA species in fallopian tube biopsy samples. Setting: Hospital and molecular genetic s laboratory. Patients: Ten women of varying ages, each presenting wit h ectopic pregnancy. Main Outcome Measure(s): Positive signal in speci fic chlamydia-directed PCR and RT-PCR assays. Result(s): Nucleic acid preparations from 7 of the 10 fallopian tube patient samples were PCR- positive for C. trachomatis DNA. Each of the 7 PCR-positive samples al so showed the presence of several transcripts from the bacterium, incl uding primary transcripts from the ribosomal RNA operons. Conclusion(s ): A higher proportion of-ectopic pregnancies than was believed previo usly may be attributable to infection of the fallopian tubes by C. tra chomatis. The presence of various chlamydial RNA molecules suggests th at viable, metabolically active bacteria were present in fallopian tub es of the patients studied. (Fertil Steril(R) 1998;70:945-8. (C) 1998 by American Society for Reproductive Medicine.).