INVESTIGATION OF MYCOBACTERIOPHAGE MTPH11 HOST-CELL INTERACTION BY ELECTRON-MICROSCOPY, FLUOROMETRY, AND ELECTROORIENTATIONAL SPECTROSCOPY

Authors
ZHILENKOV EL SHEMYAKIN IG FOMCHENKOV VM IVANOV AY GAVRYUSHKIN AV OBOROTOV MV
Citation
El. Zhilenkov et al., INVESTIGATION OF MYCOBACTERIOPHAGE MTPH11 HOST-CELL INTERACTION BY ELECTRON-MICROSCOPY, FLUOROMETRY, AND ELECTROORIENTATIONAL SPECTROSCOPY, Microbiology (New York), 67(5), 1998, pp. 550-554
Citations number
9
Categorie Soggetti
Microbiology
Journal title
Microbiology (New York) → ACNP
ISSN journal
00262617
Volume
67
Issue
5
Year of publication
1998
Pages
550 - 554
Database
ISI
SICI code
0026-2617(1998)67:5<550:IOMMHI>2.0.ZU;2-X
Abstract
According to electron-microscopic data, various cells in the Mycobacte rium smegmatis ATCC607 population interact differently with phage MTPH 11. Fluorometric studies of phage-host interactions were performed usi ng a membranotropic fluorescent probe, 8-anilino-1-naphthalene sulfona te (ANS). Changes in the electric characteristics of mycobacterial cel ls infected with the phage were studied by electro-orientational (EO) spectroscopy. The problem of the employment of fluorometry and EO spec troscopy for rapid phage typing of mycobacteria is discussed.