CAPILLARY ISOELECTRIC-FOCUSING WITH ANIONIC COATED CAPILLARIES

Capillary isoelectric focusing (cIEF) is a powerful analytical techniq ue for the separation of proteins. cIEF can be performed in capillarie s with reduced electroosmotic flow, or in capillaries with controlled electroosmotic flow. This work introduces an alternative method for cI EF with controlled electroosmotic flow through the use of an anionic c oated capillary. The method is shown to be applicable to basic, neutra l and acidic proteins, with some peak broadening for acidic proteins. It also proves to have good reproducibility with R.S.D.s around 3%, a linear relationship between migration time and isoelectric point and q uantitative capability. The total analysis time is shorter and sample preparation is simplified over other cIEF methods.