DEVELOPMENT AND APPLICATION OF THE ANALYTICAL METHODS WITH SPECTROMETRY FOR BIO-RELATED COMPOUNDS

Recent studies on the development and application of analytical method s in HPLC with spectrometric detection for bio-related compounds have been reviewed. First, new fluorescent derivatization reagents with ben zofurazan skelton have been developed. In the course of the study, the relationship between fluorescent characteristics and substituent grou ps of benzofurazan compounds was investigated. The relationship enable d to design new fluorogenic reagents easily Secondly, the simultaneous determination method for the sequence and D/L configuration of peptid es with fluorescent Edman reagent was developed. In this study, the ra cemization mechanism of amino acids in the cleavage/cyclization step w as investigated and boron trifluoride was employed as cleavage/cycliza tion reagent in order to supress the racemization. Finally, sensitive and selective determination system for catecholamine with peroxyoxalat e chemiluminescence detection after derivatization with ethylenediamin e was developed. Utilizing this system, plasma catecholamine level of spontaneously hypertensive rats and Wistar-Kyoto rats after administra tion of calcium antagonists were measured, and the attenuation of the baroceptor reflex was investigated.