A lysC-lac'Z fusion plasmid was constructed to study the regulatory re
gion of the lysC gene. Analysis by deletion mutations confirmed the ex
istence of an alternative promoter, P2, located upstream of the previo
usly identified promoter, P1. The transcription start site of promoter
P2 was located 85 base pairs upstream the transcription start site of
promoter P1. Both promoters are regulated by lysine. (C) 1998 Federat
ion of European Microbiological Societies. Published by Elsevier Scien
ce B.V. All rights reserved.