A RAPID DETECTION METHOD FOR PAI-1 PROMOTER INSERTION DELETION POLYMORPHISM (4G/5G)/

Plasminogen activator inhibitor-1 (PAI-1) is an important inhibitor of fibrinolysis, and increased levels of PAI-1 are associated with ather oma and myocardial infarction. A common 4G/5G insertion/deletion polym orphism located in the promoter region of PAI-1 gene has been describe d associated with PAI-1 activity in plasma levels. Genotyping of this polymorphism is commonly conducted with an allele-specific oligonucleo tide melting technique. In the present study, we describe a quick, eas y method for genotyping 4G/5G polymorphism in the promoter region of t he PAI-1 gene.