Jc. Dealmeida, A 28-FOLD INCREASE IN SECRETORY PROTEIN-SYNTHESIS IS ASSOCIATED WITH DNA PUFF ACTIVITY IN THE SALIVARY-GLAND OF BRADYSIA-HYGIDA (DIPTERA, SCIARIDAE), Brazilian journal of medical and biological research, 30(5), 1997, pp. 605-614
When the first group of DNA puffs is active in the salivary gland regi
ons S1 and S3 of Bradysia hygida larvae, there is a large increase in
the production and secretion of new salivary proteins demonstrable by
[H-3]-Leu incorporation. The present study shows that protein separati
on by SDS-PAGE and detection by fluorography demonstrated that these p
olypeptides range in molecular mass from about 23 to 100 kDa. Furtherm
ore, these proteins were synthesized mainly in the S1 and S3 salivary
gland regions where the DNA puffs C7, C5, C4 and B10 are conspicuous,
while in the S2 region protein synthesis was very low, Others have sho
wn that the extent of amplification for DNA sequences that code for mR
NA in the DNA puffs C4 and B10 was about 22 and 10 times, respectively
. The present data for this-group of DNA puffs are consistent with the
proposition that gene amplification is necessary to provide some cell
s with additional gene copies for the production of massive amounts of
proteins within a short period of time (Spradling AC and Mahowald AP
(1980) Proceedings of the National Academy of Sciences, USA, 77: 1096-
1100).