Sc. Palmon et al., ESTROGEN INCREASES CGMP IN SELECTED BRAIN-REGIONS AND IN CEREBRAL MICROVESSELS, Journal of cerebral blood flow and metabolism, 18(11), 1998, pp. 1248-1252
We have previously reported that exogenous and endogenous estrogen can
amplify residual cerebral blood flow during experimental cerebral isc
hemia. Because estrogen has been linked to nitric oxide and cyclic gua
nosine monophosphate (cGMP) signaling in noncerebral tissue, we tested
the hypothesis that long-term 17 beta-estradiol treatment increases b
asal cGMP in brain homogenates and cerebral microvessels in female rab
bits. We also determined whether there are important baseline gender-s
pecific differences in regional cGMP. Adult female rabbits were implan
ted with 17 beta-estradiol pellets, 10 mg (F10, n = 10) or 50 mg (F50,
n = 13), and compared with untreated females (F, n = 19) and males wi
th negligible estrogen (M, n = 19) (plasma 17 beta-estradiol levels of
4 +/- 4 pg/mL in M, 7 +/- 5 pg/mL in F, 141 +/- 74 pg/mL in F10, and
289 +/- 110 pg/mL in F50). Cyclic GMP was determined by radioimmunoass
ay in cerebellum, hypothalamus, caudate nucleus, hippocampus, and cort
ex. Cerebral microvessels were harvested from additional cohorts of un
treated males and females or estradiol-implanted females (n = 6 per gr
oup). Basal cGMP was higher in F versus M only in cerebellum. Estrogen
-induced increases in regional cGMP were prominent in hippocampus at a
ll doses (M = 43 +/- 26, F = 43 +/- 21, F10 = 84 +/- 24, F50 = 117 +/-
55 fmol/mg protein) and in cortex at the high dose (M = 78 +/- 55, F
= 88 +/- 51, F10 = 69 +/- 34, F50 = 143 +/- 52 fmol/mg protein). Simil
arly, microvascular cGMP increased only in females treated with the 50
mg dose (M = 77 +/- 13, F = 86 +/- 25, F10 = 106 +/- 35, F50 = 192 +/
- 88 fmol/mg protein). Therefore, 17 beta-estradiol increases cGMP con
tent in parenchymal regions that are known physiologic targets for rep
roductive steroids but are also areas of selective vulnerability to is
chemic insult. Further, high doses of estrogenic steroids could amplif
y cGMP signaling within the cerebral microvasculature.