STUDIES OF SOME BIOLOGICAL AND PHYSICOCHEMICAL PROPERTIES OF HERPES-SIMPLEX VIRUS TYPE-2 GROWTH-FACTOR (HSGF-2)

Herpes simplex virus type 2 (HSV-2) growth factor (HSGF-2) consists of three components. HSGF-2, and HSGF-2, components have an ability to t ransform cultured cells in vitro, whereas the HSGF-2, component change s the transformed phenotype towards the normal one. For production of functional HSGF-2, glucose and Ca2+ ions are required. The replacement of glucose by some other monosaccharides, or the presence of three cy tostatics in the medium of HSGF-2 producing MK-2 cell influenced both the biological activity and the component characteristics of HSGF-2. H eating, low pH, or UV light elevated the transforming, but not the tra nsformed phenotype repressing activity of HSGF-2. Heparin destroyed th e suppressing effect on transformed phenotype, without any influence o n transforming activity of HSGF-2. All presented results are compared with similar or different results obtained with pseudorabies virus gro wth factor (PRGF).