Z. Zivicova et al., STUDIES OF SOME BIOLOGICAL AND PHYSICOCHEMICAL PROPERTIES OF HERPES-SIMPLEX VIRUS TYPE-2 GROWTH-FACTOR (HSGF-2), ACT VET B, 67(3), 1998, pp. 159-165
Herpes simplex virus type 2 (HSV-2) growth factor (HSGF-2) consists of
three components. HSGF-2, and HSGF-2, components have an ability to t
ransform cultured cells in vitro, whereas the HSGF-2, component change
s the transformed phenotype towards the normal one. For production of
functional HSGF-2, glucose and Ca2+ ions are required. The replacement
of glucose by some other monosaccharides, or the presence of three cy
tostatics in the medium of HSGF-2 producing MK-2 cell influenced both
the biological activity and the component characteristics of HSGF-2. H
eating, low pH, or UV light elevated the transforming, but not the tra
nsformed phenotype repressing activity of HSGF-2. Heparin destroyed th
e suppressing effect on transformed phenotype, without any influence o
n transforming activity of HSGF-2. All presented results are compared
with similar or different results obtained with pseudorabies virus gro
wth factor (PRGF).