AN OPTOSENSOR FOR TRYPTOPHAN WITH C18 SILICA-GEL AS A SUBSTRATE

Authors
GONG ZL ZHANG ZJ
Citation
Zl. Gong et Zj. Zhang, AN OPTOSENSOR FOR TRYPTOPHAN WITH C18 SILICA-GEL AS A SUBSTRATE, Fresenius' journal of analytical chemistry, 357(8), 1997, pp. 1097-1100
Citations number
23
Categorie Soggetti
Chemistry Analytical
Journal title
Fresenius' journal of analytical chemistry → ACNP
ISSN journal
09370633
Volume
357
Issue
8
Year of publication
1997
Pages
1097 - 1100
Database
ISI
SICI code
0937-0633(1997)357:8<1097:AOFTWC>2.0.ZU;2-U
Abstract
A flow-through optosensor for tryptophan with C18 silica gel as a subs trate is proposed. It has been developed in conjunction with a flow-in jection analysis system and is based on the retention of tryptophan on the C18 column and the enhancement of its fluorescence. The detection limit is 25 ng mL(-1) with a relative standard deviation of 2.9% for 7 determinations of 10 mu g mL(-1) of tryptophan. Most of the common s pecies do not interfere. The method has been successfully applied to a pharmaceutical preparation.