NEUROTRANSMITTER RELEASE FROM SEMIINTACT SYNAPTOSOMES

Authors
TANDON A TAN PK BANNYKH S BANERJEE A BALCH WE
Citation
A. Tandon et al., NEUROTRANSMITTER RELEASE FROM SEMIINTACT SYNAPTOSOMES, Methods (San Diego, Calif., Print), 16(2), 1998, pp. 198-203
Citations number
35
Categorie Soggetti
Biology,"Biochemical Research Methods
Journal title
Methods (San Diego, Calif., Print) → ACNP
ISSN journal
10462023
Volume
16
Issue
2
Year of publication
1998
Pages
198 - 203
Database
ISI
SICI code
1046-2023(1998)16:2<198:NRFSS>2.0.ZU;2-#
Abstract
We have developed a secretion assay composed of semi-intact synaptosom es from which transmitter release is optimally evoked by micromolar Ca 2+ in the presence of cytosol. Transmitter release from this preparati on reconstitutes known characteristics of regulated exocytosis and is accompanied by a marked decrease in synaptic vesicles. The assay is us eful in characterizing the components known to be involved in transmit ter release, and should also facilitate the identification of addition al factors that are important for this process. (C) 1998 Academic Pres s.