OVEREXPRESSION OF THE FNR PROTEIN OF ESCHERICHIA-COLI WITH T7 EXPRESSION SYSTEM

Authors
STUCHLIK S TURNA J
Citation
S. Stuchlik et J. Turna, OVEREXPRESSION OF THE FNR PROTEIN OF ESCHERICHIA-COLI WITH T7 EXPRESSION SYSTEM, Folia microbiologica, 43(6), 1998, pp. 601-604
Citations number
13
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Folia microbiologica → ACNP
ISSN journal
00155632
Volume
43
Issue
6
Year of publication
1998
Pages
601 - 604
Database
ISI
SICI code
0015-5632(1998)43:6<601:OOTFPO>2.0.ZU;2-V
Abstract
We have used the T7 expression system for expression of E. coil FNR pr otein. The fnr gene was cloned from its initiation codon ATG into the NdeI site of an expression vector and filamentous phage mCP1-2 was use d as a donor of T7 RNA polymerase gene. The level of FNR expression at tained by this expression arrangement was about 45 % of total cell pro teins.