The nucleotide sequence encoding the citrate synthase (CS) gene was de
termined from the sequencing of the CS cDNA isolated from a human hear
t cDNA library. The primary sequence of CS deduced from its nucleotide
sequence reveals a highly conserved, albeit slightly larger, protein
of 466 amino acids, with 95% homology to its pig homologue. The data a
lso indicate that the human genomic CS gene contains no introns, and c
onfirms the location of the human CS gene on chromosome 12.