Jc. Luft et al., IDENTIFICATION AND CHARACTERIZATION OF THE TUMOR-SUPPRESSOR P53 IN CHANNEL CATFISH (ICTALURUS-PUNCTATUS), Comparative biochemistry and physiology. B. Comparative biochemistry, 120(4), 1998, pp. 675-682
Herein is presented the sequence of a catfish full-length p53 cDNA obt
ained from a cloned B cell line cDNA library. Southern blot analyses d
etermined that a restriction fragment linked polymorphism (RFLP) exist
ed with PstI among outbred catfish. Western blot analyses demonstrated
that, when compared to PBLs, the catfish leukocyte lines express high
er levels of p53 protein. Additionally, the results of Western blot an
alyses and in vitro translation experiments suggest that the catfish l
eukocyte lines may produce truncated forms of p53 due to internal init
iation. (C) 1998 Elsevier Science Inc. All rights reserved.