CD95-MEDIATED APOPTOSIS - NO VARIATION IN CELLULAR-SENSITIVITY DURINGCELL-CYCLE PROGRESSION

Sensitivity of CD95-mediated apoptosis has been reported to vary durin g cell cycle progression (FEBS Lett. (1997) 412, 91-93), Here, we repo rt that three human glioma cell lines with different p53 status (i) un dergo growth arrest and synchronous cell cycle re-entry after prolonge d serum deprivation, (ii) do not exhibit cell cycle-related changes in CD95 expression at the cell surface, and (iii) do not exhibit cell cy cle-related changes in susceptibility to DC95 ligand-induced apoptosis , In contrast, cell cycle-specific activity was demonstrated for vario us cancer chemotherapy drugs, Further, CD95 expression and susceptibil ity to CD95 ligand-induced apoptosis does not vary during cell cycle p rogression of Jurkat T cells, HeLa cervical carcinoma and HepG2 hepato cellular carcinoma cells. These results do not support a role for the cell cycle phase as an important predictor of vulnerability to CD95-me diated apoptosis, (C) 1998 Federation of European Biochemical Societie s.