CLONING OF THE CDNA-ENCODING THE LARGE SUBUNIT OF HUMAN RNASE HI, A HOMOLOG OF THE PROKARYOTIC RNASE HII

Citation
P. Frank et al., CLONING OF THE CDNA-ENCODING THE LARGE SUBUNIT OF HUMAN RNASE HI, A HOMOLOG OF THE PROKARYOTIC RNASE HII, Proceedings of the National Academy of Sciences of the United Statesof America, 95(22), 1998, pp. 12872-12877
Citations number
37
Categorie Soggetti
Multidisciplinary Sciences
ISSN journal
00278424
Volume
95
Issue
22
Year of publication
1998
Pages
12872 - 12877
Database
ISI
SICI code
0027-8424(1998)95:22<12872:COTCTL>2.0.ZU;2-U
Abstract
Two RNases H of mammalian tissues have been described: RNase HI, the a ctivity of which was found to rise during DNA replication, and RNase H II, which may be involved in transcription. RNase HI is the major mamm alian enzyme representing around 85% of the total RNase H activity in the cell. By using highly purified calf thymus RNase HI we identified the sequences of several tryptic peptides, This information enabled us to determine the sequence of the cDNA coding for the large subunit of human RNase HI. The corresponding ORF of 897 nt defines a polypeptide of relative molecular mass of 33,367, which is in agreement with the molecular mass obtained earlier by SDS/PAGE. Expression of the cloned ORF in Escherichia coli leads to a polypeptide, which is specifically recognized by an antiserum raised against calf thymus RNase HI. Intere stingly, the deduced amino acid sequence of this subunit of human RNas e HI displays significant homology to RNase HII from E, coli, an enzym e of unknown function and previously judged as a minor activity. This finding suggests an evolutionary link between the mammalian RNases HI and the prokaryotic RNases HII, The idea of a mammalian RNase HI large subunit being a strongly conserved protein is substantiated by the ex istence of homologous ORFs in the genomes of other eukaryotes and of a ll eubacteria and archaebacteria that have been completely sequenced.