HIV-1 TAT PROTEIN MIMICRY OF CHEMOKINES

The HIV-1 Tat protein is a potent chemoattractant for monocytes, We ob served that Tat shows conserved amino acids corresponding to critical sequences of the chemokines, a family of molecules known for their pot ent ability to attract monocytes, Synthetic Tat and a peptide (CysL(24 -51)) encompassing the ''chemokine-like'' region of Tat induced a rapi d and transient Ca2+ influx in monocytes and macrophages, analogous to beta-chemokines, Both monocyte migration and Ca2+ mobilization were p ertussis toxin sensitive and cholera toxin insensitive. Cross-desensit ization studies indicated that Tat shares receptors with MCP-1, MCP-3, and eotaxin, Tat was able to displace binding of beta-chemokines from the beta-chemokine receptors CCR2 and CCR3, but not CCR1, CCR4, and C CR5, Direct receptor binding experiments with the CysL(24-51) peptide confirmed binding to cells transfected with CCR2 and CCR3. HIV-1 Tat a ppears to mimic beta-chemokine features, which may serve to locally re cruit chemokine receptor-expressing monocytes/macrophages toward HIV p roducing cells and facilitate activation and infection.