S. Yamaguchi et al., DEVELOPMENTAL-CHANGES IN EXPRESSION OF ANGIOTENSINOGEN MESSENGER-RNA IN RAT NEPHRON SEGMENTS, HYPERTENS R, 21(3), 1998, pp. 155-161
We studied the localization of angiotensinogen mRNA in rat nephron seg
ments and the differences in angiotensinogen mRNA levels between male
Sprague-Dawley rats at 6 and 12 wk of age using reverse transcription
and polymerase chain reaction (RT-PCR). Each nephron segment of the ra
t kidney was microdissected. Total RNA was prepared and used in the fo
llowing RT-PCR assay. The PCR products were size-fractionated by agaro
se gel electrophoresis, visualized with ethidium bromide staining, and
identified by Southern blot analysis. The relative amounts of product
s were determined by densitometry. Strong bands corresponding to angio
tensinogen mRNA were detected from proximal convoluted and straight tu
bules, and weaker bands were found in glomeruli. The signals in all ti
ssues in 12-wk-old rats were weaker than those in 6-wk-old rats. Since
local angiotensinogen is the unique substrate of the tissue renin-ang
iotensin system and exerts an autocrine-paracrine influence on renal f
unction, the changes in tubular angiotensinogen may be related to phys
iological and morphological changes in the rat kidney during developme
nt.