K-RAS GENE-MUTATIONS IN THE DIAGNOSIS OF FINE-NEEDLE ASPIRATES OF PANCREATIC MASSES - PROSPECTIVE-STUDY USING 2 TECHNIQUES WITH DIFFERENT DETECTION LIMITS

Citation
J. Mora et al., K-RAS GENE-MUTATIONS IN THE DIAGNOSIS OF FINE-NEEDLE ASPIRATES OF PANCREATIC MASSES - PROSPECTIVE-STUDY USING 2 TECHNIQUES WITH DIFFERENT DETECTION LIMITS, Clinical chemistry, 44(11), 1998, pp. 2243-2248
Citations number
22
Categorie Soggetti
Medical Laboratory Technology
Journal title
ISSN journal
00099147
Volume
44
Issue
11
Year of publication
1998
Pages
2243 - 2248
Database
ISI
SICI code
0009-9147(1998)44:11<2243:KGITDO>2.0.ZU;2-Y
Abstract
Detection of K-ms mutations may be useful in the evaluation of pancrea tic cancer. The aim of this study was to assess, in a prospective desi gn, the diagnostic utility of K-ras mutation analysis in 62 consecutiv e fine-needle aspirates of pancreatic masses, using two PCR-based tech niques-standard and enriched-with detection limits of a mutant allele in the presence of 10(2) or 10(3) wild-type alleles, respectively. Cyt ology alone offered a diagnostic sensitivity of 75%. The enriched high er sensitivity detection technique, in combination with cytology, offe red a diagnostic sensitivity of 91% without false positives. The molec ular analysis would have contributed to diagnosis in an additional 14 cases of pancreatic cancer. The standard technique contributed to diag nosis in an additional 9 cases. These results strongly support the use of the enriched method of detecting K-uns mutations as a complement t o cytology in the evaluation of pancreatic masses.