Bha. Maas et al., LYOPHILIZED BOVINE HEMOGLOBIN AS A POSSIBLE REFERENCE MATERIAL FOR THE DETERMINATION OF HEMOGLOBIN DERIVATIVES IN HUMAN BLOOD, Clinical chemistry, 44(11), 1998, pp. 2331-2339
We investigated the suitability of a lyophilized bovine hemoglobin (LB
H) preparation containing various fractions of oxyhemoglobin (O(2)Hb),
carboxyhemoglobin (COHb), and methemoglobin (MetHb) for quality asses
sment in multicomponent analysis (MCA) of hemoglobin derivatives. It w
as demonstrated that a stable preparation of these components after re
constitution yields a hemoglobin solution that is spectrophotometrical
ly equivalent with a fresh bovine hemoglobin solution. The preparation
was found to be stable for at least 1 year when it is kept at 2-8 deg
rees C and for 1 h after reconstitution. We determined the fractions o
f O(2)Hb, COHb, and MetHb of several LBH preparations, using the compl
ete spectra of 480-650 nm with 2-nm intervals and absorptivities as de
termined for pure LBH solutions. A field trial involving various types
of multiwavelength hemoglobin photometers showed the suitability of L
BH as a quality-control material. Computer models of the various commo
n multiwavelength hemoglobin photometers may be useful for establishin
g more accurate target values of LBH preparations for each type of pho
tometer and for studying the importance of the influence of specific f
actors such as wavelength selection, absorptivity values, and interfer
ing dyes.