REMOVAL OF SPECIFIC PROTEIN SPOTS ON THE PATTERNS OF NONDENATURING 2-DIMENSIONAL ELECTROPHORESIS USING PROTEIN-A AGAROSE AND ANTIBODIES

Protein spots, such as immunoglobulin G (IgG), A (IgA) or alpha(2)-mac roglobulin, on the patterns of non-denaturing two-dimensional polyacry lamide gel electrophoresis (2-D PAGE) were selectively removed by the application of protein A or antibody;conjugated protein A agarose to t he first-dimension gel top at the cathode side. A protein residing at pI 5.8 and with a molecular mass of about 200 kDa was clearly detected when the IgG and IgA spots were removed from the 2-D PAGE pattern. (C ) 1998 Elsevier Science B.V. All rights reserved.