POLYACRYLAMIDE CONTAINING SUGAR RESIDUES - SYNTHESIS, CHARACTERIZATION AND CELL COMPATIBILITY STUDIES

Hydrophilic polyacrylamide compounds having glucose (PAAm-glucose) and galactose (PAAm-galactose) as pendent groups were synthesized from po ly(acryloyl chloride) by polymer analogous reactions. The degree of gl ucose and galactose substitution was 53.2 and 41.6%, respectively. Tis sue culture polystyrene (TCPS) plates coated with these polymers showe d increased surface wettability. FTIR-ATR spectra of coated plates sho wed characteristic bands at 1658, 1716 and 1734 cm(-1) due to the carb onyl groups of polyacrylamide, PAAm-glucose and PAAm-galactose, respec tively. Coating of polyacrylamide, PAAm-glucose and PAAm-galactose ont o TCPS plates was also confirmed by X-ray photoelectron spectroscopic (XPS) characterization. Rat hepatocytes in primary culture attached to the surfaces of PAAm-glucose and PAAm-galactose, but did not attach t o those of polyacrylamide (PAAm). Only small differences were found be tween PAAm-glucose, PAAm-galactose and PAAm in the attachment of L929 and CHO-K1 cells. Cell growth rate behavior of the latter two cell lin es on PAAm-glucose, PAAm-galactose and PAAm was almost identical. (C) 1998 Elsevier Science Ltd. All rights reserved.