NEW SPECTROPHOTOMETRIC PROCEDURE FOR DETERMINING CEFOTAXIME BASED ON DERIVATIZATION WITH 1,2-NAPHTHOQUINONE-4-SULFONATE INTO SOLID-PHASE EXTRACTION CARTRIDGES - APPLICATION TO PHARMACEUTICAL AND URINE SAMPLES

Authors
MARTINEZ LG CAMPINSFALCO P SEVILLANOCABEZA A BOSCHREIG F
Citation
Lg. Martinez et al., NEW SPECTROPHOTOMETRIC PROCEDURE FOR DETERMINING CEFOTAXIME BASED ON DERIVATIZATION WITH 1,2-NAPHTHOQUINONE-4-SULFONATE INTO SOLID-PHASE EXTRACTION CARTRIDGES - APPLICATION TO PHARMACEUTICAL AND URINE SAMPLES, Journal of chromatography B. Biomedical sciences and applications, 718(1), 1998, pp. 143-151
Citations number
30
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
718
Issue
1
Year of publication
1998
Pages
143 - 151
Database
ISI
SICI code
0378-4347(1998)718:1<143:NSPFDC>2.0.ZU;2-M
Abstract
Cefotaxime was derivatised with 1,2-naphthoquinone-4-sulphonate (NQS), extracted into solid-phase cartridges (C-18) and detected using a UV- visible detection system. Optimum conditions for this new procedure we re: hydrogencarbonate-carbonate buffer, pH 10.5, 5-min reaction time a t 25 degrees C and an NQS concentration of 7.1.10(-3) mol l(-1). The a ccuracy and the precision of the liquid-solid procedure were tested. T he procedure was used to measure cefotaxime in pharmaceutical and urin e samples. The results obtained were contrasted with those reported fo r a HPLC method for urine samples. The generalized H-point standard ad ditions method was used to measure cefotaxime in urine samples. (C) 19 98 Elsevier Science B.V. All rights reserved.