F. Sczesny et al., CAPILLARY ELECTROPHORETIC DRUG-MONITORING OF METHOTREXATE AND LEUCOVORIN AND THEIR METABOLITES, Journal of chromatography B. Biomedical sciences and applications, 718(1), 1998, pp. 177-185
Citations number
43
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
High-dose methotrexate is an important element of treatment protocols
in childhood acute lymphocytic leukemia or osteosarcoma. A capillary e
lectrophoretic method has been developed to measure peak levels and th
e metabolite pattern in patients with delayed methotrexate elimination
. It serves to determine plasma levels of methotrexate, leucovorin and
their metabolites, 7-hydroxymethotrexate, 2,4-diamino-N-10-methylpter
oic acid and 5-methyltetrahydrofolic acid. For the determination of hi
gh concentrations (>10 mu M) protein precipitation by acetonitrile wil
l suffice for sample preparation. All other samples undergo solid-phas
e extraction and upgrading on C-18 columns. Aminopterin, a therapeutic
antecedent of methotrexate, serves as internal standard. Detection is
done on a UV detector with a 300 nm filter. Five hundred mu l of seru
m are needed to determine 0.2 mu M of a specified substance (0.5 mu M
5-methylterahydrofolic acid) with good precision and accuracy. For pea
k levels, 20 mu l of capillary serum are sufficient. (C) 1998 Elsevier
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