TIME-COURSE OF SDS-ALKALINE LYSIS OF RECOMBINANT BACTERIAL-CELLS FOR PLASMID RELEASE

SDS-alkaline lysis of recombinant Escherichia coil cell suspensions wa s carried out in a coaxial cylinder rheometer, and the data were used to establish the time course of lysis reaction. The results of the exp eriments showed that cell lysis reaction time depended on cell strain but was unaffected by plasmid size and plasmid copy number. The high m olecular weight globular proteins and chromosomal DNA were denatured, and the resulting changes in rheometric measurements characterised the denaturation time. (C) 1998 John Wiley & Sons, Inc.