DNASE I HYPERSENSITIVE SITES IN THE CHROMATIN OF THE CHICKEN MSX2 GENE DIFFER IN ANTERIOR AND POSTERIOR LIMB MESENCHYME, CALVARIAL OSTEOBLASTS AND EMBRYONIC FIBROBLASTS
Z. Pan et al., DNASE I HYPERSENSITIVE SITES IN THE CHROMATIN OF THE CHICKEN MSX2 GENE DIFFER IN ANTERIOR AND POSTERIOR LIMB MESENCHYME, CALVARIAL OSTEOBLASTS AND EMBRYONIC FIBROBLASTS, Biochemistry and molecular biology international, 46(3), 1998, pp. 549-557
To identify candidate cis-acting regulatory regions involved in regula
tion of the Msx2 gene in anterior limb mesenchyme and calvarial osteob
lasts, DNase I hypersensitive sites (DHSs) from -6.1 kb to +8.4 kb rel
ative to the translation start site of the chicken Msx2 gene were iden
tified in anterior and posterior limb mesenchyme, calvarial osteoblast
s, and embryonic fibroblasts. A total of 12 DHSs were detected. Except
for the DHS in the basal promoter region, none of the other DHSs were
present in all four tissues, suggesting that the chromatin structure
in the Msx2 gene locus is differently organized in these four cell typ
es. One DHS was unique to Msx2 expressing cells and a second site to n
onexpressing cells. Anterior and posterior limb mesenchyme had similar
patterns of DHSs that were much more complex than observed in calvari
al osteoblasts possibly reflecting differences in the complexity of Ms
x2 regulation in these two tissues.