PURIFICATION AND CHARACTERIZATION OF A FERULOYLESTERASE FROM ASPERGILLUS-AWAMORI

Authors
KOSEKI T FURUSE S IWANO K MATSUZAWA H
Citation
T. Koseki et al., PURIFICATION AND CHARACTERIZATION OF A FERULOYLESTERASE FROM ASPERGILLUS-AWAMORI, Bioscience, biotechnology, and biochemistry, 62(10), 1998, pp. 2032-2034
Citations number
16
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
10
Year of publication
1998
Pages
2032 - 2034
Database
ISI
SICI code
0916-8451(1998)62:10<2032:PACOAF>2.0.ZU;2-D
Abstract
A feruloylesterase was purified from the extracellular broth of Asperg illus awamori grown on wheat bran culture. The purified enzyme gave a single band on SDS-polyaclylamide gel electrophoresis and isoelectric focusing, with an apparent M-r of 35,000 and a pI of 3.8, respectively . The substrate specificity of the purified enzyme differed obviously from that of acetylesterase of A. awamori. The enzyme bound to microcr ystalline cellulose.