SUCCESSFUL ARTIFICIAL-INSEMINATION FOR INDOOR BREEDING IN THE JAPANESE MONKEY (MACACA-FUSCATA) AND THE CYNOMOLGUS MONKEY (MACACA-FASCICULARIS)

Methods of artificial insemination (AI) for indoor breeding in the Jap anese monkey and the Cynomolgus monkey were investigated. For the Japa nese monkey AI was carried out in six females during the winter mating season and in six females during the summer non-mating season. During the mating season, semen was inseminated near ovulation time in natur al menstrual cycles. In the mating season study, three females insemin ated at the uterine cavity became pregnant. Three inseminated at the c ervical canal failed to become pregnant. For the non-mating season stu dy, ovulation was induced artificially by PMSG and hCG and AI was carr ied out near the induced ovulation time. In the non-mating season, no animals became pregnant. Of four Cynomolgus monkeys used, pregnancy oc curred in two animals inseminated near ovulation time in natural menst rual cycles. AI occurred at the uterine cavity in one and cervical can al in the other. In both species ovulation was verified by laparoscopy . Semen was collected by penile electro-stimulation then diluted to 2. 5 to 5.0x 10(7)/ml with Whitten's medium, Diluted semen of 0.2ml was i nseminated at the uterine cavity or cervical canal. Our results indica te the usefulness of vaginal Al as a method of artificial indoor breed ing.